your query. Underlining links throughout the website. Streets, S. Weiss, X. Michalet, High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins, Methods 169, 21 (2019). M. Mamenko, O. Zaika et al., Ca2+ Imaging as a Tool to Assess TRP Channel Function in Murine Distal Nephrons, Methods Mol. Generation of ATTO-labeled -syn. -ATTO 550 carboxy MSDS. the pleasure of joining the flow cytometry community with our acquisition of Flow Cytometry Standards Corporation (FCSC) in 2000. . 0000213629 00000 n S. Baliga, C. Murphy, L. Sharon, S. Shenoy, D. Biranthabail, H. Weltman, S. Miller, R. Ramasamy, J. Shah. Flavin nucleotides are the primary issue in flow cytometry laboratories because those molecules are excited in the cyan-blue range (430-500 nm) of the color spectrum, which is where the flow cytometer's primary lasers emit light (488 nm). Yang, E. Cooper, B. Chen, K. Siminovitch, A. Peterson. These percentages are automatically calculated for each compound currently on the graph. For longer periods, small aliquots should be stored at -20C. The strongest evidence for the participation of GPC3 in carcinogenesis stems from studies on hepatocellular carcinoma and lung squamous cell carcinoma. Recombinant fragment within Mouse ASIC1 aa 450-550 (C terminal). 0000190962 00000 n J.S. Flow cytometry is a lab test used to analyze characteristics of cells or particles. The insulin receptor (IR) binds insulin and IGFs with different affinities triggering different cell responses. This affords greater application flexibility allowing you to easily move assays from one platform to another. Protect from light. ATTO-TEC GmbH Martinshardt 7 D-57074 Siegen Germany Phone: +49 271 23853 - 0 FAX: +49 271 23853 - 11 E-mail: info@atto-tec.com http: www.atto-tec.com Revised: 2022-12-13 ATTO 550 is a novel fluorescent label related to the well-known dye Rhodamine 6G. Not for use in diagnostic or therapeutic procedures. After choosing a subject in the menu, waiting time is necessary for the page to upload. Antibody concentration after reconstitution. A core lab workhorse providing power, performance and consistency. 0000186873 00000 n Victoria Power Station, During the process, a sample of cells or particles is suspended in fluid and injected into a flow cytometer machine. Powerful, modern technologies built into the MA900 system include a patented micro fluidic chip-based design, comprehensive fluidic controls, and advanced automation that dramatically simplifies operation to make sorting less subjective and improve reliability. Flow cytometry studies are used to identify and quantify immune cells and characterize hematological malignancies.1 They can measure: cell size. Maximum absorption 554 nm; maximum fluorescence 576 nm. Chem. Mater Interfaces 3, 4812 (2011). ]2*1{O~8]~sC1mPf6W")D42ng,RN`U&dyNq3oLo| Northland College Women's Hockey Roster, Sumita, M.R. This application claims benefit under 35 U.S.C. Figure 7. 0000008615 00000 n Spectra Viewer. BD and the BD Logo are trademarks of Becton, Dickinson and Company. 65 0 obj <>stream EP2211174A2 EP10158606A EP10158606A EP2211174A2 EP 2211174 A2 EP2211174 A2 EP 2211174A2 EP 10158606 A EP10158606 A EP 10158606A EP 10158606 A EP10158606 A EP 10158606A EP 2211174 A2 EP2211174 A2 EP 2211174A2 Authority EP European Patent Office Prior art keywords particles polymer particles multicolored heterogenous dyes Prior art date 2005-07-11 Legal status FIG. 550 575 Details Vybrant DyeCycle Green: 506 534 Details Vybrant DyeCycle Orange: 519 . Anti-STIM1 (extracellular) Antibody (#ACC-063) is a highly specific antibody directed against an extracellular epitope of the human Stromal interaction molecule 1. Luke Summer House Ex Girlfriend, If our products do not perform as stated, please contact our technical support staff. Atto 647N is an extraordinary highly fluorescent dye, and Atto 655 are alternatives to Cy5 and Alexa Fluor 647. Western blot analysis (unlabeled antibody, Subscribe Newsletters and Email Updates, STIM1 (extracellular) Blocking Peptide (#BLP-CC063), Anti-CRACR2A (EFCAB4B) Antibody (#ACC-324), Anti-Human Orai1 (extracellular) Antibody (#ACC-060), Anti-Human Orai1 (extracellular)-FITC Antibody (#ACC-060-F), Anti-Orai1 (extracellular) Antibody (#ACC-062), Anti-Orai1 (extracellular)-ATTO Fluor-488 Antibody (#ACC-062-AG), Anti-SLC35G1 (extracellular) Antibody (#ANT-196), Anti-STIM1 (extracellular) Antibody (#ACC-063), Anti-TMEM66-ATTO Fluor-594 Antibody (#ACC-067-AR), Immunohistochemistry (IHC) Protocols for Paraffin-embedded Sections, Immunocytochemistry (ICC) Protocols for Fixed or Live Cells: Indirect and Direct Methods. When Did The Hamburger Become Popular, Y. Jiang, A. Matevossian, H.-S. Huang, J. Straubhaar, Sch. E. Favaro, D.R. This page has been recently translated and is available in French now. 0000004578 00000 n Phone: 305-822-0666 Images were taken as two or four channel images at a . New fluors Epithelial sodium channel (-subunit, neoepitope) monoclonal antibody (11-35-1) Factor XII (human, heavy chain) monoclonal antibody (10-11-37) Discover all new products It is alternately an exc CF450 Dye. Marktgasse 18 8302 Kloten - Svizzera Tel: +41 (044) 805 76 81 Fax: +41 (044) 805 76 75 E-mail: contactus@anawa.ch Applications: Bioactivity. C 114, 4345 (2010). Tomov et al., Detailed Study of DNA Hairpin Dynamics Using Single-Molecule Fluorescence Assisted by DNA Origami, J. Phys. F. Dake, Y. Taki, Time-domain fluorescence lifetime imaging by nonlinear fluorescence microscopy constructed of a pump-probe setup with two-wavelength laser pulses, Applied Optics 57, 757 (2018). Chem. Telefon: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: orders@antikoerper-online.de ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. Herten, G. Nette, G. Schenk, M. Seefeld, Is CuII Coordinated to Patellamides inside Prochloron Cells?, Chemistry - A European Journal (2017). ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescent quantum yield, and high photo-stability. - maleimide Claude, A. Moreau, J. Lumeau, J. Wenger, Extending Single-Molecule Frster Resonance Energy Transfer (FRET) Range beyond 10 Nanometers in Zero-Mode Waveguides, ACS Nano 13, 8469 (2019). J. Wardyn, A. Chan, A. Jeyasekharan, A Robust Protocol for CRISPR-Cas9 Gene Editing in Human Suspension Cell Lines, Current Protocols 1, e286 (2021). With over 35 years of research, development, and manufac- Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO) is directly labeled with an ATTO-550 fluorescent dye. 18, 523 (2008). When Did The Hamburger Become Popular, Ffx Qactuar Monster Arena, L. Dirix, K. Kennes, E. Fron, Z. Debyser, M. van der Auweraer, J. Hofkens, S. Rocha, Photoconversion of Far-Red Organic Dyes, ChemPhotoChem 2, 433 (2018). As the fluorescing cell passes through the laser beam, it creates a peak or pulse of photon emission over time. Rua Almada Negreiros Lote 5, Loja 14 2615-275 Alverca do Ribatejo - Portugal Tel. Chromatin Immunoprecipitation (ChIP) can be technically challenging and yield results that are difficult to interpret. W. Ren, S. Wen, S. Tawfik, Q. Su, G. Lin, L. Ju, M. Ford, H. Ghodke, A. van Oijen, D. Jin. Spectra varies slightly from lot to lot. The fluorescence is excited most efficiently in the range 575 610 nm. To view a known dye, scroll down the menu on the left and click to select. Sc., President and Technical Director, Omega Optical is a leader in photonics, Expression of KV1.5 in mouse cerebellum - Immunohistochemical staining of perfusion fixed free-floating frozen mouse brain sections usingAnti-KV1.5 (KCNA5)-ATTO-550 Antibody(#APC-004-AO) (1:50) (red). 0000029289 00000 n D. Kozak, A. Chen, M. Trau, Profiling Protein-Surface Interactions of Multicomponent Suspensions via Flow Cytometry, Langmuir 24, 1204 (2008). 0000238713 00000 n M. Baibakov, S. Patra, J.-B. S. Mukherjee, J.-M. Knop, S. Mbitz, R. Winter, Alteration of the Conformational Dynamics of a DNA Hairpin by -Synuclein in the Presence of Aqueous Two-Phase Systems, Chemistry A European Journal 26, 10987 (2020). Atto 550 is spectrally similar to. Lawrie et al., Flow cytometric detection of proteolysis in peptide libraries synthesised on optically encoded supports, Mol. JMRK%\Of&-:\m;DQ8_T,9EXeM'gJ Qi^Fd-j*E65l) }%$%o^? A one parameter histogram plotting channel number vs. number of events. Maximum absorption 601 nm; Maximum fluorescence 627 nm. JavaScript is not activated in your browser. J. Scholefield, R. Henriques, A. Savulescu, E. Fontan, A. Boucharlat, E. Laplantine, A. Smahi, A. Isral, F. Agou, M. Mhlanga, Super-resolution microscopy reveals a preformed NEMO lattice structure that is collapsed in incontinentia pigmenti, Nature Communications 7, 12629 (2016). Flow cytometry is unique in its ability to measure, analyze, and study vast numbers of homogenous or heterogeneous cell populations. . Technical Support, Order Information ATTO 550 is a cationic dye. For Research Use Only. Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO) is directly labeled with an ATTO-550 fluorescent dye. 19% tax excl. 0000022708 00000 n Alexa Fluor and Texas Red are trademarks of Life Technologies Corporation. Converse Library Sample, Grning et al., A molecular toolkit for population genetic investigations of the ash dieback pathogen Hymenoscyphus pseudoalbidus, For. Tregs are known to express the highest levels of CD25. All other trademarks are the property of their respective owners. 0000002570 00000 n ATTO 550 Orange 554 576 791 Cy3.5 Orange Red 581 596 1,286 microscopy, flow cytometry and immunohistochemistry. LCI- Live cell imaging, N- Neutralization, WB- Western blot, For research purposes only, not for human use. Recently, we demonstrated that GNF-2, an allosteric c . 0000186798 00000 n R.-L. Troskie, Y. Jafrani, T. Mercer, A. Ewing, G. Faulkner, S. Cheetham, Long-read cDNA sequencing identifies functional pseudogenes in the human transcriptome, Genome Biology 22, 1 (2021). Chem. The XN-550 features an automated sampler and so improves workflow productivity with its Rerun & Reflex functionality and continuous loading feature. 0000002382 00000 n 0000007840 00000 n 85, 7753 (2013). Maximum absorption 554 nm; maximum fluorescence 576 nm. 0000196811 00000 n 0000276147 00000 n Luke Summer House Ex Girlfriend, Atto 550 is a fluorescent compound with an excitation peak at 554 nm and an emission peak at 575 nm. A fluorophore free in solution may have a different quantum yield than the same fluorophore attached to a protein, which in turn also depends on the extent of protein-to-fluorophore labeling (1-3). J. Nikolic, L. Belot, H. Raux, P. Legrand, Y. Gaudin, A. Albertini. Overview of Flow Cytometry Reagents Mix-n-Stain Antibody Labeling Kits Apoptosis Assays Dead Cell Stains Proliferation & Viability Assays Cell Cycle Analysis Flow Cytometry Accessory Products View all in Flow Cytometry BACK Overview of CF Dyes & Other Bioconjugates Annexin V Conjugates Alpha Bungarotoxin Conjugates W. Ren, S. Wen, S. Tawfik, Q. Su, G. Lin, L. Ju, M. Ford, H. Ghodke, A. van Oijen, D. Jin, Anisotropic functionalization of upconversion nanoparticles, Chemical Science 9, 4352 (2018). - streptavidin P2Y receptors can be divided into two main subgroups: the P2Y 1 subfamily which comprises P2Y 1, P2Y 2, P2Y 4, P2Y 6, and P2Y 11 receptor subtypes. Second, to optically distinguish and quantify intracellular cholesterol accumulation, we have adapted the classical filipin cholesterol staining protocol. M. Pagliaro, R. Ciriminna, M. Wong Chi Man, S. Campestrini, Better Chemistry through Ceramics: The Physical Bases of the Outstanding Chemistry of ORMOSIL, J. Phys. Infected cells were then analyzed and quantified through MACS flow cytometry (Miltenyi Biotec). BD flow cytometers are Class I (1) laser products. Alternatively, you can always add additional dump channels by clicking the button again. If you are having trouble resolving a population in a channel, especially one close to a laser line, it may be worth investigating a laser light leakage issue into that channel. C. Kim, O.-c. Lee, J.-Y. Starbound Weapon Tiers, Recently, several key players of the store-operated complex have been identified.3The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. S. Patra, M. Baibakov, J.-B. Several possible interactions between STIM1 and Orai1 have been suggested. c/o Carr Riggs Ingram, 500 Grand Boulevard, Suite 210 Miramar Beach, FL 32550 - USA Tel: +1 850 650 7790 Fax: +1 850 650 4383 E-mail: info@biotrend-usa.com The probe was labeled with the Atto-550 dye. Adding a dump channel to your panel design is easy! Bode Plot Solved Examples In Control System Pdf, A. Borgia, M. Borgia, K. Bugge, V. Kissling, P. Heidarsson, C. Fernandes, A. Sottini, A. Soranno, K. Buholzer, D. Nettels, B. Kragelund, R. Best, B. Schuler. Atto 594 is an alternative to Alexa Fluor 594 and Texas Red. BD FACSDiva Software controls the efficient setup, acquisition and analysis of flow cytometry data from the BD LSRFortessa Workstation. W. Ye, M. Gtz, S. Celiksoy, L. Tting, C. Ratzke, J. Prasad, J. Ricken, S. Wegner, R. Ahijado-Guzmn, T. Hugel, C. Snnichsen, Conformational Dynamics of a Single Protein Monitored for 24 h at Video Rate, Nano letters 18, 6633 (2018). endstream endobj startxref 2023 BD. M. Busby, L. K. J. Stadler et al., Optimisation of a multivalent Strep tag for protein detection, Biophys. T.E. Starbound Weapon Tiers, Flow cytometry combines the flexibility and sensitivity of fluorescence technology with high speed and data integration capabilities. D. Bracha, M. Walls, M.-T. Wei, L. Zhu, M. Kurian, J. Avalos, J. Toettcher, C. Brangwynne. Within our portfolio, we gladly take on special requests for: Customized antibody labeling HlWI$7+>0z@ARAK ~IFdU4.!Sc)IS 13 ~_\ /I G-r,qS^V(s5OqpTFZg^}wHI)e&aO(RKLp_IH!A` ZlP Rp2bw )Ps9{ To detect far-red fluorescence in cells labeled with the Alexa Fluor 647 or Cy5 A set of polymer particles stained with at least two fluorescent dyes is presented. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Looks like you're visiting us from {{countryName}}. Effects of heme proteins on nitric oxide levels and cell viability in isolated PMNs: a mechanism of toxicity RichardA. T` GDbqb~Jh!7}IXc-tOa^ A. Andreoni, L. Nardo, R. Rigler, Time-resolved homo-FRET studies of biotin-streptavidin complexes, Journal of Photochemistry and Photobiology B: Biology 162, 656 (2016). These molecules are very useful in flow cytometry, because of their brightness, they excite well with the typical 488 nm laser line, and can serve as efficient FRET donors to near-infrared dyes. In high-throughput mode, the HTS option can speed through a 96-well plate in less than 15 minutes with less than 0.5% sample carryover from one well to the next. - ATTO 550 absorption (.txt), Absorption and Emission Spectrum (graphic) Additional file 4: Figure S4 Internalization of FITC-Insulin and BAC-Ins-SA-atto 550. Rat, mouse, bovine and canis - identical. X. Hopkins, W. Gill, R. Kringel, G. Wang, J. Hass, S. Acharya, J. 0000190721 00000 n 0000002015 00000 n The Atto series includes Atto 390, Atto 425, Atto 465, Atto 488, Atto 550, Atto 633, Atto 647N, and Atto 680. M. Urban, S. Both, C. Zhou, A. Kuzyk, K. Lindfors, T. Weiss, N. Liu. Search 0 ATTO 550 Antibodies Search Antibodies Open Interactive . Flow cytometry was used to determine T cell phenotype and ion channel expression. 49913 - ET - 633-640nm Laser Longpass Set for AlexaFluor 647, DyLight 649, Atto 647: 49914 - ET - 640-647nm Laser Bandpass for Set AlexaFluor 647, DyLight647, Atto 647N: 49915 - ET - 355-375nm Laser Longpass Set for Uncaging and Ablation: 49916 - ET - Shortpass Filter Set for 1064nm Laser Tweezing or CARS Beam Combining By using the right combination of channel exposed 200ms, green channel exposed 800ms. BUV395 is designed for instruments equipped with a 355 nm UV laser and a 379/28 filter. The website has an accessibility menu. Endoplasmic reticulum stress activates inositol-requiring enzyme 1 (IRE1) and protein kinase, R-like endoplasmic reticulum kinase (PERK), the two principal regulators of the unfolded protein response (UPR). We offer 100% guarantee on all our products. Marks, A. de Magis, H. Kazemier, D. Hilbig, D. Benhalevy, X. Wang, M. Hafner, K. Paeschke, DHX36 prevents the accumulation of translationally inactive mRNAs with G4-structures in untranslated regions, Nature Communications 10, 2421 (2019). Surawsky, S.R. N. Gilat, D. Torchinsky, S. Margalit, Y. Michaeli, S. Avraham, H. Sharim, N. Elkoshi, C. Levy, S. Zirkin, Y. Ebenstein, Rapid Quantification of Oxidation and UV Induced DNA Damage by Repair Assisted Damage Detection-(Rapid RADD), Analytical Chemistry 92, 9887 (2020). 0000270695 00000 n L. Cruz, T. van Dijk, O. Vepris, T. Li, T. Schomann, F. Baldazzi, R. Kurita, Y. Nakamura, F. Grosveld, S. Philipsen, C. Eich, PLGA-Nanoparticles for Intracellular Delivery of the CRISPR-Complex to Elevate Fetal Globin Expression in Erythroid Cells, Biomaterials 268, 120580 (2021). Northland College Women's Hockey Roster, Santa Cruz Biotechnology now offers a broad range of Stains, Dyes and Fluorescent Probes categorized by their Excitation and Emission Values. 555, ATTO 550, Cy3, DyLight 549, Rhodamine. Dy-550, Alexa Fluor 555, and Atto 565 dyes have . Kang, J. Jose, J.-C. Pyun, Screening of biotin-binding FV-antibodies from autodisplayed FV-library on E. coli outer membrane, Analytica Chimica Acta 1169, 338627 (2021). Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune Flow Cytometers. - iodoacetamide The software is common across BD FACS instrument families, including the BD FACSCanto Cell Analyzer andBD FACSAria Cell Sorter systems. Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates The dye is moderately hydrophilic. Spark Blue 550, Spark NIR 685) are only recommended for use in multicolor spectral flow cytometry panels. Atto 550 can be used with similar excitation source and fluorescence filters as Cy3 and is characterized by a high photostability. R. H. Meltzer, J. R. Krogmeier et al., A lab-on-chip biothreat detection using single-molecule DNA mapping, Lab Chip 11, 863 (2011). She, R. Tornay, E. Leimgruber, D. Bernasconi, L. Lagopoulos, P. Renaud, N. Demierre, P. van den Bogaard, Rapid, sensitive and real-time multiplexing platform for the analysis of protein and nucleic-acid biomarkers, Analytical Chemistry 87, 1582 (2015). 0000128088 00000 n The CD61-ImmunoPLT reference method was performed on the FC-5000 flow cytometer . Would you like to stay on the current country site or be switched to your country? Luke Summer House Ex Girlfriend, This dye is highly suitable for direct flow cytometry (FACS) using the He:Ne laser. 0000223495 00000 n of lymphocytes and other cells, owing to its ability to make quant., homogeneous, multiparameter measurements of particles. Warrantee of use applies to the website owners and/or their representative, including the content displayed in the website, as subject to the conditions of use. C.R. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN. These plots represent data taken from a BD LSRFortessa System using an 8-color panel that applies this gating strategy to identify FoxP3-positive cells. U. Chio, S. Chung, S. Weiss, S.-O. D. Daems, W. Pfeifer, I. Rutten, B. Sacc, D. Spasic, J. Lammertyn. H. Mnck, D. Toppe, E. Michael, S. Sigrist, V. Richter, D. Hilpert, D. Raccuglia, M. Efetova, M. Schwrzel. The fluorescence is excited most efficiently in the 540 - 565 nm range. 51, 8 (2014). 2023 Alomone Labs. The fluorescence channel and relative brightness for each of the fluorochromes. Email: info@cdltmds.com, CopyRight 2018 CDL Technical & Motorcycle Driving School, Hours of Service (Log Books) 8 Hours Certification Course, CMV Driver Knowledge & Skills Evaluation 6 Hours Certificatrion Course, CDL 6 Hours Preparation Course Class B-Truck, P-Bus, S-Bus, CDL 10 Hours Preparation Course Class A, B-Truck, P-Bus, S-Bus, COURSES CDL 20 Hours Preparation Course Class A, B-Truck, P-Bus, S-Bus, Heavy Commercial 40 Hours CDL Class A Tractor Trailer Certification Course, COURSES Light Commercial 40 Hour CDL Class B\P-Bus, S-Bus Certification Course, CDL Class A 80 Hours Intermediate Tractor Trailer Certification Course, Bode Plot Solved Examples In Control System Pdf. APC is excited by the red diode laser and excites in several tandem dyes including APC-Cy5.5 and APC-Cy7. Res. You are now leaving the BD Biosciences website. Please activate JavaScript to have access to all shop functions and all shop content. Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates The dye is moderately hydrophilic. : +32 2 31 50 800 Fax: +32 2 31 50 801 E-mail: info@kyvobio.be Flow cytometry analysis of Jurkat cells stained with CF633 Mix-n-Stain labeled mouse anti-human CD3 antibodies (BD cat# 555330). Changing color contrast based on dark backgrounds Our reagent specialists are here to help you find the best product for your application. Through the use of various reporter stains, fluorescence-based .
Adjectives To Describe Starr Carter,
Scottish Daily Mail Delivery,
Articles A
